Refer the following template DNA strand to answer the questions. 3\' TAC TGA GTA

Question

Refer the following template DNA strand to answer the questions. 3' TAC TGA GTA GGA TAC CCT AGA ATT 5' What would be the sequence of the complimentary DNA strand that would bind to the template DNA strand at the top of the page? What is the sequence of the mRNA strand that would be produced from the template DNA strand at the top of the page? What is the sequence of the amino acids that would be produced if the template DNA strand at the top of the page is converted to a protein? What would happen to the mRNA produced in problem 33 and the protein produced in problem 34 if there was a substitution mutation (C becomes G) in the second intron of the template DNA at the top of the page?

Explanation / Answer

29. The sequence of complimentary DNA strand that would bind to the template DNA starnd given at the top of the page would be,

(A-T and G-C rule)

5' ATG ACT CAT CCT ATG GGA TCT TAA 3'

30. The mRNA sequence produced from the tmplate DNA strand given at the top of the page would be,

(A-U and G-C rule)

3' AUG ACU CAU CCU AUG GGA UCU UAA 5'

31. The sequence of the amino acid that would be produced from the template DNA strand at the top of the page would be,

(N-terminus) 5' Met Thr His Pro Met Gly Ser stop 3' (C-terminus)

32. when C is relaced by G,

The mRNA sequence produced from the tmplate DNA strand given at the top of the page would be,

(A-U and G-C rule)

3' AUC ACU CAU CCU AUC CCA UCU UAA 5'

31. The sequence of the amino acid that would be produced from the template DNA strand at the top of the page would be,

(N-terminus) 5' Ile Thr His Pro Ile Pro Ser stop 3' (C-terminus)

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