510 Chemistry: Moran et al Proc. Natl. Acad. Sci. USA 94 (9970 Table 2. Accuracy

Question

510 Chemistry: Moran et al Proc. Natl. Acad. Sci. USA 94 (9970 Table 2. Accuracy of template base selection for a given nucleoside triphosphate Nucleoside Template Efficiency, triphosphate base (X) Km, HLM max, min-1 Vmax/Km Accuracy dTTP 2.5 (0.8) 43 (4) 1.7 x 10 2.200 (800) 0.39 (0.07) 1.8 x 102 1.1 X 10-5 1.5 (0.1) 2.6 x 103 1.5 x 10-4 0.079 (0.002) 4.4 x 102 2.6 x 10-5 1.2 (0.4) 7.1 x 103 4.2 x 10-4 FTP 15 (0)t 4.2 x 105t 370 (90) 0.038 (0.002) 1.0 x 10 2.4 x 10-4 190 (40) 0,0056 (0.0003) 2.9 x 101 6.9 x 10-5 0.064 (0.005) 1.5 x 102 3.6 x 10-4 53 (4) 16 3.0 x 105 7.1 x 10-1 79 (20) 0.039 (0.002) 4.9 x 102 1.2 x 10-3 Steady-state kinetic parameters are for incorporation of single nucleotides into a template-primer dupl base by the KF polymerase. Conditions: nM containing variable 7.5), 10 mM MgC12, 1 mM dithiothreitol ug/ml enzyme, 50 TrisHCI volume of 10 ul. Error limits are given in parentheses. ne serum albumin, incubated for 1-20 min at 37 C in a reaction Efficiency relative to dTTP insertion opposite A (entries 1-5) or dFTP insertion opposite A (entries 6-11) averaged from four data sets

Explanation / Answer

16.As given in table 2 the efficiencies of insertion of dTTP opposite to A,C,G,T and F are respectively

1.7*10^7, 1.8*10^2, 2.6*10^3, 4.4*10^2 and 7.1*10^3.

Now if we arrange these data from most efficient to least we'll get the following order

1.7*10^7(for A),7.1*10^3(for F), 2.6*10^3( for G), 4.4*0^2(for T), 1.8*10^2(for C).

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