Band Sizes (bp The food tested was Jiffy Corn Muffin Mix. Did your test good gen

Question

Band Sizes (bp

The food tested was Jiffy Corn Muffin Mix.

Did your test good generate a 200 bp band with GMO primer (lane 4)?

What does this tell you about the GMO status of your food?

What other information do you need to confirm the GMO status of your smaple?

How do the results of your other five PCR reactions help support or undermine your result for your test food?

Lane Sample Bands?

Band Sizes (bp

1 Sample 1: Non-GMO food control with plant primers 2 Sample 2: Non-GMO food control with GMO primers 3 Sample 3: Test food with plant primers 4 Sample 4: Test food with GMO primers 5 Sample 5: GMO positive control DNA with plant primers 6 Sample 6: GMO positive control DNA with GMO primers 7 PCR molecular weight ruler 8 Leave empty

Explanation / Answer

Sample 1: Non-GMO food control with plant primers - 455 bp

Sample 2: Non-GMO food control with GMO primers - No band

Sample 3: Test food with plant primers - 455 bp

Sample 4: Test food with GMO primers - 200 bp or no band

Sample 5: GMO positive control DNA with plant primers - 455 bp

Sample 6: GMO positive control DNA with GMO primers - 200 bp

PCR molecular weight ruler - 1000 , 700 , 500 , 200 , 100 bp

Yes our test food generated a 200 bp band with GMO primer ( lane 4)

GMO status of food is positive

We have to cinfirm other PCR reactions and whether palnt primers determine if the DNA was successfully extracted. GMO positive template must amplify PCR reactions.

The plant primers determine whether plant DNA was successfully extracted from the sample. The non-GMO food control is an indicator of false positive results, should they occur. If the non-GMO food control comes out asGMO-positive if there is a band in lane 2 it means that the PCR was contaminated at some point during processing. The
GMO-positive template control is an indicator of false negatives.

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