2. (15 points) Name the separation mechanism that best fits the following descri

Question

2. (15 points) Name the separation mechanism that best fits the following descriptions.

Ions in the sample solution bind to fixed ion sites on the surface of a polymeric stationary phase, and compete with ions that are deliberately added to the mobile phase  

Large molecules cannot penetrate the small pores of the stationary phase, but smaller molecules (within a certain size range) do penetrate and are retained to a greater extent

A substance binds very specifically to a moiety attached to the stationary phase through an enzyme-substrate or antigen-antibody interaction   

Explanation / Answer

(1) Ion exchange chromatography

Ions in the sample solution bind to fixed ion sites on the surface of a polymeric stationary phase, and compete with ions that are deliberately added to the mobile phase

(2) Size exclusion chromatography or molecular sieve chromatography or Gel filtration chromatography.

Large molecules cannot penetrate the small pores of the stationary phase, but smaller molecules (within a certain size range) do penetrate and are retained to a greater extent

(3) Affinity chromatography

A substance binds very specifically to a moiety attached to the stationary phase through an enzyme-substrate or antigen-antibody interaction  

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