Indicate the denaturing agent in the following example: a. heat or uv light b. p
ID: 519349 • Letter: I
Question
Indicate the denaturing agent in the following example: a. heat or uv light b. pH change c. organic solvent d. heavy metal ions e. agitation placing surgical instruments in a 120 degree Celsius autoclave whipping cream to make a desert topping applying tannic acid to a burn placing AgNO_3 drops in the eyes of newborns Using alcohol to disinfect a wound Match the common name of each of the following enzymes with the description of the reaction: a. dehydrogenase b. oxidase c. peptidase d. decarboxylase e. esterase f. transaminase removes hydrogen from a substrate removes CO_2 from a substrate transfers an amino (NH_2) group from an amino acid to an alpha-keto acid Mark the following characteristics of an enzyme is true or false. is a biological catalyst functions at a low pH is obtained from the diet greatly increases the rate of a cellular reaction catalyzes at a faster rate at a higher temperature functions best at mild conditions of pH 7.4 and 37 degrees Celsius lowers the activation energy of a biological reaction increases the rate of the forward reaction, but not the reverse. Essential amino acids ____. A. are the amino acids that must be supplied by the diet. B. are not synthesized by the body. C. are missing in incomplete proteins D. are present in proteins from animal sources. E. All of the above Match the names of the enzymes with the reactions below they would catalyze: a. decarboxylase b. Isomerase c. dehydrogenase d. lipase e. sucroseExplanation / Answer
Answer to Q19)
The process in which the proteins or nucleic acids lose the quaternary structure, tertiary structure and secondary structure which is present in their native state by application of external stress is known as denaturation.
a) This stress can be in the form of heat or radiations for example in the process of autoclaving instruments at 120 deg C, heat brings about denaturation. Heat can increase the kinetic energy and cause the proteins in the microbes to vibrate rapidly. This disrupts the hydrogen bonds in the microbes and causes denaturation.
b) Tannic acid lowers the pH of the burnt skin and thus prevents infectious microbes from growing in the affected areas. The protein will lose its negative charge at this lower pH for this pH is below the isoelectric point of the protein. Thus all the molecules will contain only positive charge and this be repelled from each other. Thus bringing denaturation.
c) Organic solvents such as ethanol prevents infectious microbes from growing in the affected areas. Ethanol molecules have high affinity to hydrogen bonding. Hence they can break the existing hydrogen bonds causing denaturation in proteins.
d) Few drops of solution containing heavy metal such as AgNO3 in newborn baby’s eyes prevents it from contraction of gonorrhea from the mother, which could cause blindness. This is due to the antiseptic properties of silver. Also, heavy metals can disturb the salt balance of the proteins and precipitate as insoluble protein metal salts.
e) Whipping cream is an example of denaturation by agitation where the polypeptide chains in the cream are stretched by this process until they break.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.