Dr. Sally Sue Smith just discovered the protein BAD that was found to be a criti

Question

Dr. Sally Sue Smith just discovered the protein BAD that was found to be a critical protein in lung cancer progression. This protein was found to be WT in structure and functioned similar to those who did not have cancer. Dr. Smith hypothesized that what was mutated was the promoter of the gene bad and furthermore that this mutation caused a loss of binding to a key transcription factor, a repressor, named SAD. (DO NOT COPY AND PASTE THE PREVIOUS QUESTIONS ANSWER TO THIS ONE - IT IS WRONG - PLEASE ONLY ANSWER THE QUESTION BELOW.)

**Dr. Smith has been pleased with data so far in supporting her hypothesis and now wants to know how this loss of repressor binding has impacted the accessibility to the bad promoter. How would Dr. Smith examine promoter accessibility?

Explanation / Answer

Due to mutation in promoter there is loss of binding to repressor, which cause the initiation of transcription due to binding of transcription factor to bad promoter and hence it will lead to the development or expression of lung cancer.

Accessibility and binding sites are life-stage and tissue specific. there are some transcription factors specific to the promoter.Some TFs have well-defined motifs associated with them.In this way the

Another approach is to have data on what parts of the genome each TF binds to from ChIP-seq or something similar. This data would tell us directly whether our TF has binding sites in the promoters of interest. Then we can do simple overlaps.In this way we can find out the accessibility of the promoter.

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