Calculate the missing numbers and enter them in the blanks. A decimal point must

Question

Calculate the missing numbers and enter them in the blanks. A decimal point must have a numeral in front of it.

I added 750 micro liters of TE (10 mM Tris HCl, 1mM disodium EDTA, pH 8) to a cell pellet with a volume of 250 micro liters. When the cells were resuspended, the concentration of Tris HCl was____mM and the concentration of disodium EDTA was____mM. (Include nonzero decimals.) I added 300 micro liters of 10% SDS and 50 microliters of 20 mg/ml proteinase K, and mixed everything so that the___mg of SDS and____micro grams of proteinase K could lyse all the cells. After I added 400 micro liters of 1 M sodium acetate, pH 5.1, the total volume was 1.75ml. I added 3ml of 95% isopropyl alcohol and accidently mixed it into the lysate so that the concentration of isoprpyl alcohol became_____%.

Explanation / Answer

I added 750 micro liters of TE (10 mM Tris HCl, 1mM disodium EDTA, pH 8) to a cell pellet with a volume of 250 micro liters. When the cells were resuspended, the concentration of Tris HCl was__7.5__mM and the concentration of disodium EDTA was__0.74__mM. (Include nonzero decimals.) I added 300 micro liters of 10% SDS and 50 microliters of 20 mg/ml proteinase K, and mixed everything so that the_2,200__mg of SDS and__0.74__micro grams of proteinase K could lyse all the cells. After I added 400 micro liters of 1 M sodium acetate, pH 5.1, the total volume was 1.75ml. I added 3ml of 95% isopropyl alcohol and accidently mixed it into the lysate so that the concentration of isoprpyl alcohol became__60.12___%.

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