For each of the following separations, should a cation-exchange or anion-exchang

Question

For each of the following separations, should a cation-exchange or anion-exchange column be used?

1. A mixture of I–, F–, NO3–, SO42–, and Cl–.

2. A mixture of Na , K , Ca2 , Mg2 , and Ba2 .

3. A mixture of amino acids under acidic conditions, where the pH is less than the pI of the amino acid.

For each of the following separations, should a cation-exchange or anion-exchange column be used? A mixture of IT, F, NO SO4 and CIT 3 O not enough information O cation-exchange column O anion-exchange column A mixture of Na K Ca Mg and Ba O cation-exchange column O anion-exchange column O not enough information A mixture of amino acids under acidic conditions, where the pH is less than the pl of the amino acid. O cation-exchange column O anion-exchange column O not enough information

Explanation / Answer

ANSWER

1. To separate negatively charged ions (anions) anion-exchange chromatography is used. In anion-exchange chromatography a cationic stationary phase is used. Hence option B (cation-exchange column) is correct.

2. To separate positively charged ions (cations) cation-exchange chromatography is used. In cation-exchange chromatography an anionic stationary phase is used. Option B is correct.

3. At pH lower than PI the amino acids will have a net positive charge. Hence anion-exchange chromatography will be used. In this chromatography anoinic stationary phase (Column) will be used.

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