The microtubules you measured were stabilized with taxol, but how do we know tha

Question

The microtubules you measured were stabilized with taxol, but how do we know that the

movements that you are seeing aren’t due to microtubule assembly/disassembly (termed

treadmilling)? Here’s a hint: the fluorescently labeled microtubule subunits aren’t

incorporated into the microtubule lattice evenly. There are some regions with more

labeled tubulins, and other regions with fewer labeled tubulins. If you look closely at

your movies and zoom in, you’ll notice that the microtubules have bright and dark

regions. How can you use this pattern to support the idea that the microtubules aren’t

depolymerizing?

Explanation / Answer

Microtubules, made up of and tubulin and they consists of unbranched cylinders that are hollow and long and can be up to 25 nm in diameter. Their function is to help the movement of structures in the cell.

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