You have chemically synthesized a template strand of DNA. This template strand h

Question

You have chemically synthesized a template strand of DNA. This template strand has the sequence (CT)15 (CTCTCT…repeated 15 times for an overall 30-base sequence). You have developed an in vitro transcription system that reads the above template and produces an mRNA strand of a defined sequence. You take this resulting mRNA strand and incubate it with an in vitro translation system you have developed. What will be the sequence of the peptide that results? Assume that translation of the mRNA begins with the first codon (i.e., assume that a start codon is not required to begin translation). Also, assume that if a stop codon is encountered prior to reaching the end of the mRNA, translation stops and does not resume, in which case you should enter the sequence of amino acid(s) incorporated into the peptide prior to encountering the stop codon. Enter your answer using the one-letter code of amino acids.

Explanation / Answer

The sequence of template DNA would be as below and it runs from 3' to 5' end

DNA : 3' C T C T C T C T C T C T C T C T C T C T C T C T C T C T C T 5'

mRNA : 5' G A G A G A G A G A G A G A G A G A G A G A G A G A G A.G A 3'

mRNA : 5' G A G -A G A -G A G- A G A -G A G- A G A- G A G- A G A- G A G -AG A 3'

protein: E R E R E R E R E R

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