The Pee Checker 9001 measures the concentration of urobilin (or urochrome), the

Question

The Pee Checker 9001 measures the concentration of urobilin (or urochrome), the source of the yellow color of urine. When you are dehydrated, the urobilin is less diluted with water and your urine looks more yellow; when you are hydrated the urobilin is more diluted with water and your urine looks less yellow. By measuring how much light is absorbed by a sample, and applying Beer's Law, we can determine the concentration of urobilin in the sample and, therefore, assess the user's hydration. 1. If urobilin solutions (and urine) look yellow, what is a wavelength of visible light that a urobilin solutions probably transmits (doesn't absorb strongly)? And what is a wavelength of visible light that a urobilin solution probably absorbs (doesn't transmit strongly)?

Explanation / Answer

1. The wavelength of visible light that urobilin transmits is above 550 nm (From the graph). Minimum absorbance in the above spectrum.

2. The wavelength of visible light that urobilin absorbs is around 430 nm (From the graph)- Maximum absorbance in the above spectrum.

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