cell biology 9. Two membrane preparations have been derived from the plasma memb

Question

cell biology 9. Two membrane preparations have been derived from the plasma membrane of erythrocytes and are present in a buffer solution: (1) right- side-out membrane vesicles, in which the exoplasmic leaflet of the vesicle faces the buffer (equivalent in orientation to that of the exoplasmic leaflet of the intact plasma membrane); and (2) inside-out membrane vesicles in which the exoplasmic leaflet faces the interior of the vesicles. Right aide out veses ROVs) Annexin V is a protein that binds to phosphatidylserine, one of the phospholipids present in cell membranes. Fluorescently labeled annexin V is mixed with each vesicle preparation. The vesicles are washed to remove unbound annexin V and then examined by fluorescence microscopy. Presence of annexin V is detected by green fluorescence, as shown above. What can be deduced from these data?

Explanation / Answer

Phosphatidylserine is an important phospholipid of membrane components, and it is faced towards the cytosolic side of the cell membrane. Annexin V binds to phosphatidylserine and imparts green colour to the membrane, this can be used to identify the mebrane preparations in which the exoplasmic leaflet faces the interior of the vesicles.

Based on the above data we can say that the Inside-out vesicles (IOV's) contain phosphotidylserine in their structure.

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