In reversed phase HPLC, there is a...: (a) polar solvent/non-polar column; (b) n

Question

In reversed phase HPLC, there is a...: (a) polar solvent/non-polar column; (b) non polar solvent/polar column (c) non polar solvent/non-polar column; (d) polar solvent/polar column In HPLC, the concentration of a substance can be determined by...: (a) comparison of the area under the peak produced by the substance with the areas under the peaks produced by standard solutions (b) comparison of the retention time of the substance with that of a standard (c) calculation of the retention time of the substance (d) measurement of the height of the peak produced by the substance In gas chromatography, if two solutes with short retention times co-elute (i.e are not resolved), what is the simplest way to attempt to resolve the peaks? (a) Use a higher column temperature (b) Use a lower column temperature (c) Use a longer column (d) Use a more polar solvent

Explanation / Answer

The answer is a. Polar solvent and non polar column.

High performance liquid chromatography is a separation technique. In this compound is separated using two phases mobile phase and stationary phase..

In reverse phase chromatography, mobile phase is polar and stationary phase is non polar, so that non polar part of sample binds to HPLC whereas polar part moves down the column with polar solvent during HPLC run. This technique is used to isolate polar compounds.

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