Adding ice cold 95% EaoH your solution causes DNA to podpitste, wHr The question

Question

Adding ice cold 95% EaoH your solution causes DNA to podpitste, wHr The questions below might heb answer this question. one largely oomposed of. EMctively, you havechanged the solvent tom an aqueoussoluson to ls ties new solvent mow or less polarthan the original solAion? what atractve nomaly help keep ONA insoluton? Decreasing the temperaure of the solution also helps 2 The specmum belo isfatofanetracted ONA sample from herring sperm or you were cutous). The methods foretracting the DNA were similar to those usedin hislab 2.5 2.0 1.5

Explanation / Answer

polar molecules, like DNA interact electrostatically with the water molecules, allowing them to easily dissolve in water. Because water is a polar molecule – it has a partial negative charge near the oxygen atom due the unshared pairs of electrons, and partial positive charges near the hydrogen atoms.  Nucleic acids are hydrophilic due to the negatively charged phosphate (PO3-) groups along the sugar phosphate backbone. But it is precipitate in ethanol because polarity of ethanol is very less copmpared to water but DNA is highly polar because negatively charged phosphate (PO3-) groups along the sugar phosphate backbone. This will cause precipitation of DNA in ethanol.

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