In order to decrease the retention time in ion chromatography, the analyst may (

Question

In order to decrease the retention time in ion chromatography, the analyst may (A) decrease the flow of the eluent. (B) inject less sample on the column. (C) increase the length of the column. (D) increase ionic strength of the eluent. Which is not a characteristic of capillary zone electrophoresis? (A) flat flow profile (B) separation achieved by a combination of electrophoretic and electroosmotic flow (C) capillary columns lacking stationary phase (D) required mobile phase gradient for complete separation of complex mixtures Items #39 and #40 refer to the isochratic chromatogram below, for which an absorbance detector was used. Which would explain peak A at 4.5 min having higher concentration than peak B at 6.4 min? (A) The molar absorptivity of peak A at 4.5 min is lower than that for peak B at 6.4 mm. (B) Absorbance changes with the extent of dilution due to diffusional broadening. (C) Absorbance detectors are mass sensitive. (D) Longer retained peaks exhibit greater preconcentration at the beginning of the column. What is the resolution of the A and B analytes? ÍA) 0.65 (B) 12 (C) 2.1 (D) 5

Explanation / Answer

Ans 37. D . Increase the ionic strength of the eluent

Ion chromatography seperates the ions and polar molecules based on their affinity towards the ion exchangers.

The ionic strength of the eluent is directly related to its eluting ability . Hence the time required for the seperation of ions decreases as the ionic strength increases

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