To culture bacteria for a DNA maxi-prep, you need to make 500 mL of LB broth wit

Question

To culture bacteria for a DNA maxi-prep, you need to make 500 mL of LB broth with a final concentration of 100 mu g/mL ampicillin and you have a 100 mg/mL stock solution of ampicillin. How much ampicillin do you add? Convert units to make answer measurable. A DNA sequencing service cells for 500 ng of a 7 kb of a 7 kb DNA plasmid. What volume of 0.25 mu g/mu L solution of DNA do you need to sent away? Convert units to make answer measurable. You have the following buffer recipe and decide to make up some concentrated solutions first so that you won't have to weight out dry ingredients, wait for them to dissolve, and correct the pH every time you make it, How much dry ingredients do you use for each of the following 4 stock solutions? Show your work and include units in your calculations. 150 mM NaCl (formula weight 58.44 g) 10 mM Tris pH 8.0 (formula weight 121 g) 0.15% bovine serum albumin (i) 3 M NaCl, making up 100 mL (ii) 1 M Tris, pH 8.0, making up 100 mL (iii) 10% bovine serum albumin, making up 10 mL Next, how much of each stock (listed below) plus water do you use for 400 mL of the buffer recipe above? Choose units so that your answer is measurable- e. g. not 0.05 L or 50,000 uL, but 50 mL. Show your work and include units in your calculations.

Explanation / Answer

Question 14

We will use the formula: C1V1 = C2V2

We'll make a solution V2 = 500mL and C2 = 100ug/mL.

We have a stock solution with C1 = 100mg/mL.

However we need to have the concentrations in the same units, this means we have to change C1 to ug/mL just multiplying it times 1000. So:

C1 = 100,000ug/mL

C2 = 100ug/mL

V1 = ?

V2 = 500mL

Let's solve for V1 in the equation:

(100,000 ug/mL)*V1 = (100ug/mL)*500mL

V1 = 0.5mL

So, we need to add 0.5mL of the stock solution of ampiciline.

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