Please also explain why you picked your answer. When carrying out the analysis o

Question

Please also explain why you picked your answer.

When carrying out the analysis of morphine and its metabolites in postmortem blood, solid phase extraction (SPE) is often used in the sample preparation step prior to analysis. Which ONE of the following describes the mechanism of how this preparation step is used? The SPE cartridge allows the morphine and its metabolites to remain in a suitable solvent. Morphine and its metabolites are retained on the chromatographic media of the SPE cartridge before being washed out with an appropriate solvent. The SPE cartridge acts as a simple filter, removing large particles that would interfere with the HPLC column. Morphine and its metabolites are not in the correct ionic form, therefore the SPE cartridge will allow the chemistry of the analytes to be modified for analysis via HPLC.

Explanation / Answer

16 When carrying out the analysis of morphine and its metabolites in post- mortem blood, solid phase extraction (SPE) is often used in the sample preparation step prior to analysis. Which ONE of the following describes the mechanism of how this preparation step is used?

A. The SPE cartridge allows the morphine and its metabolites to remain in a suitable solvent--ANSWER

B .Morphine and its metabolites are retained on the chromatographic media of the SPE cartridge before being washed out with an appropriate solvent.

C. The SPE cartridge acts as a simple filter, removing large particles that would interfere with the HPLC column.

D Morphine and its metabolites are not in the correct ionic form, therefore the SPE cartridge will allow the chemistry of the analytes to be modified for analysis via HPLC

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Strong stage extraction (SPE) is a specimen arrangement handle by which intensifies that are broken up or suspended in a fluid blend are isolated from different mixes in the blend as per their physical and substance properties. Systematic research centers utilize strong stage extraction to think and purge tests for examination. Strong stage extraction can be utilized to disengage analytes of enthusiasm from a wide assortment of frameworks, including pee, blood, water, drinks, soil, and creature tissue.

SPE utilizes the liking of solutes disintegrated or suspended in a fluid (known as the versatile stage) for a strong through which the specimen is passed (known as the stationary stage) to isolate a blend into coveted and undesired parts. The outcome is that either the coveted analytes of intrigue or undesired polluting influences in the specimen are held on the stationary stage. The segment that goes through the stationary stage is gathered or disposed of, contingent upon whether it contains the coveted analytes or undesired debasements. In the event that the bit held on the stationary stage incorporates the coveted analytes, they can then be expelled from the stationary stage for gathering in an extra stride, in which the stationary stage is washed with a suitable eluent

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