When a concentration of substrates increases continuously in a simple chemical r

Question

When a concentration of substrates increases continuously in a simple chemical reaction, the rate of product formation continues increasing. Is t also the case for an enzymatic reaction? Explain why? a) After obtaining 100 ml of your 100 mM (phosphate pKa_1 - 2.16, pKa_2 - 7.21 and pKa_3 - 12.32) buffer at required pH of 7.4, would you expect to change that pH by few drops of 100 mM HCI ? Explain b) What specific molecule or ion HCI is reacting with? A student obtained following plot in an enzyme inhibition assay. What type of an inhibitor he is studying?

Explanation / Answer

Ans 9.

As the rate of substrates increase in a chemical reaction , the rate of product formation also increases and hence the reaction rate .

This is not always true for enzymatic reactions.

Increase in substrate concentration would increase the product formation uptill an extent after which it will not affect the reaction rate anymore.

Enzymes have an active site in their structure , which is utilised by the substrates to form a intermediate and subsequently a product.

Hence The product concentration will be affected untill all the active sites are occupied , after which no further increase in the substrate concentration would affect product formation .

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