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Serum actually contains thousands of proteins and many of these arc of interest

ID: 53048 • Letter: S

Question


Serum actually contains thousands of proteins and many of these arc of interest to Pepole who are studying human medicine and diseases. Most scrum proteins are VERV difficult to set yb electrophoresis without further analysis. The following picture is from an advertisement commercial kit that removes some of the most abundant proteins from serum, to the benen people who want to study some of these rarer proteins. The pie chart represents the prevalence of some of the very abumdant proteins in scrum, including albumin and antibodies, among others. The small wedge on the right of the pie chart represents the large number of other proteins that are also present in the scrum. Based on what you saw with the rabbit scrum, and understanding that human is pretty much the same, why would someone want to remove the abundant proteins from serum before trying to do experiments with it? Please be as specific as possible.

Explanation / Answer

Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms.Protein purification is either preparative or analytical. Preparative purifications aim to produce a relatively large quantity of purified proteins for subsequent use. Examples include the preparation of commercial products such as enzymes (e.g. lactase), nutritional proteins (e.g. soy protein isolate), and certain biopharmaceuticals(e.g. insulin). Analytical purification produces a relatively small amount of a protein for a variety of research or analytical purposes, including identification, quantification, and studies of the protein's structure, post-translational modifications and function.Hence the Purification of Proteins Is an Essential.if there is any residual or contaminat protiens other than our required protien in experiment will give us errors.so it is always pefferable that the protien should be in pure form and only one type of molecule, in which the biochemist is interested.

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