Question 17. You intend to precipitate various proteins from plasma using ammoni

Question

Question 17. You intend to precipitate various proteins from plasma using ammonium sulfate using the table below and the table at the end of the quiz (nomogram for determining the amount of ammonium sulfate needed to attain respective percentages of saturation.). How much ammonium sulfate powder do you need to add to precipitate each one of three fractions (fibrinogen, globulins, albumins)? Complete the last column in the table below.

Solubility of plasma proteins in (NH42SO4 solutions Saturation by Protein fraction Salt required (NH4)2SO4 precipitated (g/100 m) 0-20 Fibrinogen Globulins 20-50 50-100 Albumins

Explanation / Answer

Calculate the volume of ammonium sulphate required for precipitation using the following equation.

X = (Y×V) / 1-Y

Where X is the volume of saturated ammonium sulphate, Y is the desired final concentration of ammonium sulphate ( expressed as a decimal fraction, where 1 =100%), and V is an initial volume of sample undergoing the salt cut.

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