Bioengineering. Please answer each problem fully with details. MNE 525 Homework
ID: 53295 • Letter: B
Question
Bioengineering. Please answer each problem fully with details. MNE 525 Homework 1) Suppose that Ax is the change in a colligative property of a solution -Le a property that depends on molar concentration of the solute, not on the type of solute. Ax- k where is the total molar concentration of the solute and k is constant a) Show that the ratio of C/Ax, in which C is the easily measured mass concentration of the solute, depends only on M". If we find 5x therefore, we can determine M, even for non-uniform solutes. b) Give an example of a measurement technique that measures a change in a colligative property and what it actually measures-ie, what is Ax for the measurement you describe. 2) 4Sg of protein are dissolved in 1 liter of water. An osmometer is used to measure the AT of freezing (how much the freezing solution is found to be-2.53x10" . a) What is M, for the protein? b) If instead of 45g of protein, you added 45g of CaCl, to a liter of water, what would the equilibrium freezing point of the solution be? 3) A cell with an internal osmolality of 290 mOsm is put into isotonic media (media that has 290 mOsm of solute). The media is modified by the addition of glycerol so that it contains 1.5 Molar glycerol. The glycerol has a specific gravity of 1.4746 and an M. 92.0542 a) How much glycerol, by weight, should be added to I liter of media in order to make a 1.5M (Molar) glycerol solution? Do NOT neglect the volume of glycerol in your calculation. b) What is the initial osmotic pressure across the cell membrane after the glycerol has been added? 4) Calculate the partial specifie volume of glycine from is partial molar volume of 43.2 cm (Glycine is CH2NH,COOH) 5) Based on an energy analysis, at what temperature would you expect proteins to begin to become unstable due to thermal energy? Explain. 6) a) Calculate the photon energy for a green photon b) Recognizing that chemical bonds are broken by the energy of a single photon (not by the additive energies of multiple photons) determine the wavelengths of photons that can break hydrogen bonds and covalent bonds. c) Where does UV fall in comparison to your results in (b)? What does this indicate about the bonds that are broken in DNA? Explain why you think DNA can be damaged by UV in light of your findings in (b). d) Plastic materials often degrade in sunlight. What can you conclude about plastics based on that observation? 7) Look up and describe in about one paragraph the procedure for preparing a biological sample for SEM. Describe the challenges this might pose for the study of biomolecules and living cellsExplanation / Answer
5. Proteins start to denature at 41 degree celsius. At this temperature, weak covalent bonds like Hydrogen bonds begin to get unstable and thus disrupt the functional structure of the protein. Also other non covalent interactions get disrupted. Proteins work at specific temperature as they fold into functional structure at that particular temperature only.
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