Dialysis and chromatography both can be used to separate different molecular spe

Question

Dialysis and chromatography both can be used to separate different molecular species. Which of the following features is NOT an advantage of chromatography compared to dialysis? Chromatography can distinguish between very large molecules, whereas dialysis cannot. Chromatography can separate a sample into three or more fractions each with a different protein of interest whereas dialysis separates a sample into only two fractions. Chromatography can be used to purify precipitated proteins, whereas dialysis cannot. Chromatography can be used to separate molecules on the basis of charge, whereas dialysis cannot. All of the above are valid advantages.

Explanation / Answer

Among the different methods available for separation of compounds, mixtures etc, one of the important adaptable technique is Chromatography. Due to its versatility it has wide range of applications. For the separation of complex mixtures, Chromatography is a commonly used technique which separates compounds based on their molecular weight, size polarity, ionic mobility and boiling point etc.

However Dialysis is a separation method for the separation of molecules in a solution using the principle of rate of diffusion through semipermeable membrane. It can be helpful for separation of small molecules.

Although Dialysis and Chromatography both can be used to separate different molecular species, but Chromatography has some additional advantages over dialysis.

Hence, the answer is: A5. All of the above are valid advantages

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