a gene related to human hearing encompasses 1123 nucleotides,including all its i

Question

a gene related to human hearing encompasses 1123 nucleotides,including all its introns. There is an EcoR1 restriction site 93nucleotides upstream of the gene and a second restriction site 112nucleotides downstream of the end of the gene. There are four knownmutations in this gene that can cause deafness. The mutations areall in the same region of the gene:

TCGGATTCCCAGG
AGCCTAAGGGTCC

The first T-A pair is +316

The 4 known mutations are dt-1 a transition of T to C, dt-2 atransition of G to A, dt-3 a transversion of G to T, and dt-4 atransversion of T to A

which of these mutations could cause a new restriction site forEcoR1?

Explanation / Answer

The EcoRI restriction enzyme is able to recognize the basesequence 5' GAATTC 3' so, based on the sequence you have, a transition from G to Acould create a restriction site for EcoR1 because the originalsequence is GGATTC.

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