Imagine you crossed an Arabidopsis plant heterozygous for an embryo-defective mu
ID: 53546 • Letter: I
Question
Imagine you crossed an Arabidopsis plant heterozygous for an embryo-defective mutation with several visible marker lines homozygous for the following recessive traits: yellow inflorescence (yi), transparent seed coat (tt3), and transparent seed coat combined with hairless leaves (ttg). F1 plants segregating for the embryo-defective mutation were then allowed to self-pollinate, and the resulting F2 plants were scored for the visible marker and the presence of siliques containing 25% aborted seeds. The following (real-life) results were obtained (number of F2 plants placed in each phenotypic class):
A. Which visible marker (yi, tt3, or ttg) is most distantly linked to the EMB gene?
B. Based on the Arabidopsis genetic map of “DP” mapping lines shown in class, where would you estimate this EMB gene is located, relative to the markers noted above? Do not simply note the chromosome involved.
Visible Marker
EMB Gene
YI
TT3
TTG
+ / -
+ / +
31
20
67
+ / -
+ / emb
282
146
230
vis / vis
+ / +
87
33
51
vis / vis
+ / emb
23
22
75
Visible Marker
EMB Gene
YI
TT3
TTG
+ / -
+ / +
31
20
67
+ / -
+ / emb
282
146
230
vis / vis
+ / +
87
33
51
vis / vis
+ / emb
23
22
75
Explanation / Answer
As per the table above it is clear that TT3 is most distantly linked gene.
EMB gene is located between Visible marker and TT3 gene showing + / +
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