A. \"Sticky ends\" bind to all other pieces of DNA where bases jut out from the
ID: 53660 • Letter: A
Question
A. "Sticky ends" bind to all other pieces of DNA where bases jut out from the end, as a natural repair mechanism.
B. "Sticky ends" refers to the phosphate group on the end of the DNA molecule being sticky to the next nucleotide.
C. "Sticky ends" contain a single-stranded end complementary to the target fragment's single-stranded end with which it can pair.
D. "Sticky ends" join each other forming a circular molecule that is then clonable.
2.
Match the following terms.
1. Enzymes capable of cleaving DNA at specific sequences.
2. A piece of DNA that carries a recombinant DNA molecule and can replicate in a host cell.
3. Specific DNA sequences that are cleaved by particular restriction enzymes.
4. A single DNA molecule made from two different sources.
5. Catalyzes the formation of a phosphodiester bond between adjacent PO4 and -OH groups of nucleotides.
6. A region of a plasmid that contains a number of unique restriction sites.
A. multiple cloning site (MCS)
B. DNA ligase
C. restriction sites
D.recombinant DNA
E. vector
F. restriction endonucleases
3. Recombinant DNA is taking a single sticky ended DNA, ligating it into a circle, which can then be transformed into a host cell to be replicated.
True
False
4. Restriction endonucleases digest unused bacterial DNA to recycle the nucleotides.
True
False
5. When "sticky ends" of two DNA molecules are paired, their sugar-phosphate backbones can be permanently rejoined by
A. nucleases.
B. restriction enzymes.
C. Taq polymerase.
D. DNA ligase.
E. reverse transcriptase.
6. Which of the following is NOT one of the steps required to clone a piece of DNA using a plasmid vector?
A. Isolate DNA fragments by gel electrophoresis.
B. Transform ligated pieces of DNA into E. coli.
C. Select the plasmid vector that has replaced its origin of replication with insert DNA.
D. Cut a vector and an insert with a specific restriction enzyme to create complementary "sticky ends."
E. Insert DNA with similar sticky ends together, and ligate into vector.
7. The technique used to separate DNA molecules based on their length/mass is called:
A. restriction enzyme analysis.
B. gel electrophoresis.
C. forensic restriction digestion chain reaction.
D. Ligation construction differential analysis.
8. Which of the following characteristics is unique to YACs and BACs and is NOT true of plasmid vectors?
A. Drug selection is used to select for cells containing recombinant DNA.
B. YACs and BACs require live cells for replication.
C. X-gal screening can be used to identify clones containing inserts.
D. The YACs and BACs can accommodate very large DNA insertions.
E. DNA can be inserted into it at specific sites using a multiple cloning site (MCS).
9.
Match the following
1. An enzyme isolated from retroviruses that can make cDNA from an mRNA template.
2. A collection of many different stretches of DNA in a vector that together represents the entire genome of an organism.
3. A collection of many different types of DNA that each represents the genes expressed as proteins in many different tissues or cells.
4. Annealing of a complementary single-stranded probe to identify specific DNA's in complex mixtures.
1. reverse transcriptase
2. genomic library
3. hybridization
4. cDNA Library
10. How is a cDNA library different from a genomic library?
A. the cDNA library contains sequences not found in the genomic library.
B. cDNA contains RNA nucleotides.
C. cDNA does not contain introns.
D. cDNA libraries can be screened by hybridization while genomic libraries cannot.
E. cDNA is not double stranded while genomic DNA is.
11. A representative genomic library of an organism's overlapping DNA fragments results from the use of
A. viruses that cleave the DNA into random pieces.
B. random pieces of DNA that are made such that they never overlap.
C. DNA ligase joining multiple types of vectors.
D. many pieces of recombinant DNA from several different types of organisms.
E. partial digestion with frequently cutting restriction endonucleases.
12. In screening for clones containing specific DNA genomic library pieces, it is common to use
A. solutions that will always maintain cellular membrane integrity.
B. complementary viruses.
C. antibiotics.
D. milipore filters randomly impregnated with dyes or radiation.
E. molecular hybridization with probes
13. When wishing to create an expression library that will express proteins from a eukaryotic cell, in a bacterial host, using mRNA and reverse transcriptase solves the problem of
A. transformation of foreign DNA.
B. alternative splicing.
C. post-translational modifications.
D. nucleic acid base pair matching.
14. Which of the following is an example of reverse genetics?
A. observing a phenotype and isolating the gene that produces that phenotype
B. mutating a cloned gene and substituting the mutant copy for the wild-type copy in an organism to assess its function
C. identifying a protein-protein interaction by a yeast two-hybrid screen
D. finding a mouse gene can direct eye formation in Drosophila
E. isolating clones from a library using hybridization of a radiolabeled probe
15. One of the most useful methods for identifying a specific DNA sequence is
A. the Western blot.
B. magnetic resonance imaging.
C. the Southern blot.
D. thin-layer chromatography.
E. the Eastern blot.
16.
Match the following molecular techniques.
1. Point mutations found in a population that can eliminate or create restriction sites.
2. Allows the amplification of a single DNA fragment from a small amount of a complex mixture of DNA.
3. A technique in which proteins are separated by electrophoresis and probed with a specific antibody.
4. Acts as a chain terminator when incorporated into the DNA because it lacks an OH at the 3' position.
A. polymerase chain reaction
B. dideoxynucleotides
C. western blot
D. restriction fragment length polymorphisms
1. Why are "sticky ends" of DNA sticky?Explanation / Answer
Answer 1)
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