This is a diagram of a typical eukaryotic gene that encodes a single RNA product
ID: 53696 • Letter: T
Question
This is a diagram of a typical eukaryotic gene that encodes a single RNA product. The diagram relates an mRNA's structure to its corresponding gene. Each "D" represents 100 DNA nucleotides, while each "R" represents 100 RNA nucleotides. There is an correspondence between the two where they overlap. The translation start and stop sites are indicated. Dashes (-) connect RNA nucleotides that are connected to each other in the mature mRNA (e.g. the last nucleotide of the first "R" in the diagram is bonded to the first nucleotide of the second "R.") Italicized letters above the gene indicate the positions of point mutational sites.
Suggestion: Review the function of the translational start and stop sequences, if needed.
How many introns does this gene have? (My answer 4 )
Is the 5' end of the gene to the left or to the right? (Left)
Which of the lettered mutational sites could most likely represent a promoter mutation? a
Which of the lettered mutational sites would be most likely to affect polyadenylation of the mRNA? h
ef al DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD (gene) R---RRRR----RR--RR-RRRRRRRR--RR-R (mRNA) stop startExplanation / Answer
How many introns does this gene have?
Three introns
Is the 5' end of the gene to the left or to the right?
5' RRRRRRRRRRRRRR 3' Start from left
Which of the lettered mutational sites could most likely represent a promoter mutation?
Promotor site is the initiation of transcription (start site), exactly on top of which there is no lettered mutation seen.
Which of the lettered mutational sites would be most likely to affect polyadenylation of the mRNA?
Polyadenylation is the poly A tail at the 3' end of the mature mRNA, mutational site should be 'g'
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