Which of the following is not associated with the folding of a protein into its
ID: 537718 • Letter: W
Question
Which of the following is not associated with the folding of a protein into its native conformation?
options:
Molecular chaperones prevent folding intermediates from aggregating.
Folding of proteins is a relatively slow process as the stabilization of the molecular structure depends solely on salt bridge interactions which take time to form.
The major driving force for protein folding is the overall increase in solvent entropy. This increase in solvent entropy is a result of the strong interactions between water molecules that lead to the association of nonpolar side chains and subsequent collapse of the molecule into a more compact shape.
Hydrogen bonds contribute to the cooperativity of folding and help stabilize the native conformations of proteins.
All of the above are associated with the folding of a protein into its native conformation.
2 . A protein of interest needs to be separated from a mixture of proteins for amino acid sequencing. It is known that the protein of interest is highly enriched in lysine residues compared to the other proteins in the mixture. Which of the following techniques will be most effective in isolating this protein?
options:
gel-filtration chromatography
SDS-polyacrylamide gel electrophoresis
mass spectrometry
cation exchange chromatography
anion exchange chromatography
a)Molecular chaperones prevent folding intermediates from aggregating.
b)Folding of proteins is a relatively slow process as the stabilization of the molecular structure depends solely on salt bridge interactions which take time to form.
c)The major driving force for protein folding is the overall increase in solvent entropy. This increase in solvent entropy is a result of the strong interactions between water molecules that lead to the association of nonpolar side chains and subsequent collapse of the molecule into a more compact shape.
d)Hydrogen bonds contribute to the cooperativity of folding and help stabilize the native conformations of proteins.
e)All of the above are associated with the folding of a protein into its native conformation.
Explanation / Answer
Ans :-1 ) Hydroge bond contribute to the cooperavity of folding and help stabilize the native conformatio of protein is not associated with the folding of a protein into its native conformation because these are weak forces of interaction which is responsible for entropy increase or decrease and do not provide any contribution to foldig of protein. Therefore the correct option is D.
Ans2) The technique by which protei of interest like lysine can be separated from a mixture of protein for amino acid sequencing will be cation exchange chromatography because lysine is a positive charge amino acid which is soluble in water and thus forms ionic bond as they are opposite in charge so they will produce cation at the desired phase forming amino acid sequence.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.