need help on the X\'S.. You need to create 5 serial Dilutions of Creatinine stan

Question

need help on the X'S..

You need to create 5 serial Dilutions of Creatinine standards with a total volume of 1 mL each. You are provided with a 25 mg/dL stock Creatinine solution. The first concentration required is provided in the table below. Create a series of dilutions by cutting the concentration by 40% each time. Complete the following table based on the use of the information above to make the following PPM concentrations of Creatinine. (1 mL of total volume is used) (Give answers to 3 sigfigs):

Explanation / Answer

Series of dilution

Dilution #1,

Creatine ppm = 23.5 mg/dL/0.1 = 235 ppm

Volume of creatine needed = 23.5 x 1 ml x 1000/25 = 940 uL

Volume of saline buffer needed = 1000 - 940 = 60 uL

Dilution #2,

Creatine ppm = 14.1 mg/dL/0.1 = 141 ppm

Volume of creatine needed = 14.1 x 1 ml x 1000/25 = 564 uL

Volume of saline buffer needed = 1000 - 564 = 436 uL

Dilution #3,

Creatine ppm = 8.46 mg/dL/0.1 = 846 ppm

Volume of creatine needed = 8.46 x 1 ml x 1000/25 = 338.4 uL

Volume of saline buffer needed = 1000 - 338.4 = 661.6 uL

Dilution #4,

Creatine ppm = 5.07 mg/dL/0.1 = 507 ppm

Volume of creatine needed = 5.07 x 1 ml x 1000/25 = 202.8 uL

Volume of saline buffer needed = 1000 - 202.8 = 797.2 uL

Dilution #5,

Creatine ppm = 3.04 mg/dL/0.1 = 304 ppm

Volume of creatine needed = 3.04 x 1 ml x 1000/25 = 121.6 uL

Volume of saline buffer needed = 1000 - 121.6 = 878.4 uL

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