Gelatin Hydrolysis Test 1. Some microbiologist recommend incubating this medium

Question

Gelatin Hydrolysis Test

1. Some microbiologist recommend incubating this medium at 37 degrees C, along with an incoculating control, and then transferring all tubes to the refrigerator prior to reading them. Why might this be preffered technique in some situations? What potential problems can you see with this method?

2. If the control is solid and an incoluate tube is liquid, is it acceptable to read the result before the complete incubation time has elapsed? Why or why not?

3. If the control is solid and a tube is also solid, is it acceptable to read the result before the complete incubation time has elapsed? Why?

Explanation / Answer

1)

Transferring to the refrigerator is often used to stop grow without killing the microbes. This would be good if you could take your readings for the media at any time. Some culture media do not keep their color/change in the refrigerator. Some media also need to be read in a certain time frame, so putting them in the refrigerator could mess up your readings.

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