At time 0, an Hfr strain (strain 1) was mixed with an F- strain, and at various

Question

At time 0, an Hfr strain (strain 1) was mixed with an F- strain, and at various times after mixing, samples were removed and agitated to separate conjugating cells. The cross may be written as:
Hfr 1: a+ b+ c+ d+ e+ f+ g+ h+ Str^S
F: a- b- c- d- e- f- g- h- Str^R
No order is implied in the above listing. The samples were then plated onto selective media to measure the frequency of h+ Str^R recombinants that had received certain genes from the Hfr cell. The following graph shows the percentage of recombinants against time in minutes.
What is the order of a+, b+, e+, g+, and h+ in the Hfr chromosome, starting from the origin?

Explanation / Answer

the number of recombinants increases with the length of time of mating .

so the order is h+ ,e+, b,+ g +, a+

a + enters last after 16 minute

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