You are studying a membrane protein, Lippy, that you suspect binds to lipids wit

Question

You are studying a membrane protein, Lippy, that you suspect binds to lipids with a role in maintaining proper membrane lipid composition. You want to know both, (i) WHERE in the cell the Lippy protein resides, and WHAT is the lipid composition of the same membrane where Lippy likely localizes. Of the experimental methods listed below, which single method would be the best choice initially to use toward understanding both the identity of the membrane compartment AND the molecular composition of the membrane itself where Lippy localizes? [Select only the one best answer that could be used for both questions, not just one or the other.] Immunofluorescence microscopy Genetic analysis Live cell imaging using GFP Electron microscopy Cell fractionation

Explanation / Answer

Immunofluorescence microscopy is the best method to understand the identity of the membrane compartment.

Hence, the correct option is (A) Immunofluorescence microscopy.

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