Native Hemoglobin A is a hetero-tetramer of two alpha subunits and two beta subu

Question

Native Hemoglobin A is a hetero-tetramer of two alpha subunits and two beta subunits. Dr. Noah Lott isolated a preparation of pure alpha subunit (alpha and beta subunits are each a single polypeptide chain of about 16.000 molecular weight). Which of the following when mixed will form precipitates? polyclonal serum to alpha subunit + alpha subunit: monoclonal antibody to alpha subunit + alpha subunit; polyclonal antibody to beta subunit + alpha subunit; monoclonal antibody to beta subunit + native Hemoglobin: monoclonal antibody to alpha subunit + polyclonal antibody to alpha subunit. EXPLAIN

Explanation / Answer

a) Polyclonal serum to alpha subunit + alpha subunit = It will form precipitate because the polyclonal serum to alpha subunit will also have the antigens against alpha subunit.

b) monoclonal antibody to alpha subunit + alpha subunit = It will form precipitate. The monoclonal antibodies are prepared using a single cell line, it will essentially have the antigens against alpha subunit.

c) Polyclonal serum to beta subunit + alpha subunit = It will not form a precipitate because the polyclonal serum to beta subunit will not have the antigens against alpha subunit.

d) monoclonal antibody to beta subunit + native hemoglobin = It will form a precipitate. The monoclonal antibodies for beta subunit will bind to beta subunits in the hemoglobin.

e) monoclonal antibody to alpha subunit + polyclonal antibody to alpha subunit = It will not form any precipitate as both are antibodies.

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