The main uses of TLC in a research laboratory are to monitor reactions (old spot
ID: 545625 • Letter: T
Question
The main uses of TLC in a research laboratory are to monitor reactions (old spot(s) disappear and new spot(s) appear) and as a prelude to many other liquid chromatographic techniques that purify larger amounts of compound.
For example, determining which solvent mixture gives analytes an Rf of 0.25 saves a lot of time, effort, and money because too high of an Rf makes compound come out too quickly and too low an Rf means that compounds will appear to never come out. The former means that compounds are not separated, the latter means that too much solvent and money are wasted.
Match each description and picture to a type of liquid chromatography used in a research lab.
Used to separate volatile liquids by boiling point, affinity to the stationary phase or even size (depending on the column type), this chromatographic technique can be used to monitor reactions or even to separate the components of complex aromas so that a human can smell them at the outlet (pictured top-center left). This technique is often used in forensic analysis.
Uses are air pressure to force analytes and solvents through a long pad of the same material used in TLC plates. Capable of separating grams of compound.
Uses a high pressure pump to force analytes and solvent through micron size stationary phase material. Depending on the diameter of the column used, allows to monitor a reaction mixture or to separate mg (prep-column) of compound. A = pump, B = Injector, C =solvents, D = guard column, E = chromatography column, F = UV-Vis detector, G = computer readout, H = helium to degas the solvents
Uses a large glass backed TLC plate and a streak of mixture at the origin line to separate out mg of compound after developing the plate, scrapping off the stationary phase, and extracting out the desired compound.
Uses the same material as used in TLC plates and centrifugal force to collect up to a gram of compound on the edge of its plate.
A) High Performance Liquid Chromatography
B) Preparative Chromatography
C) Gas Chromatography
D) Radical Chromatography
E) Flash Chromatography
Used to separate volatile liquids by boiling point, affinity to the stationary phase or even size (depending on the column type), this chromatographic technique can be used to monitor reactions or even to separate the components of complex aromas so that a human can smell them at the outlet (pictured top-center left). This technique is often used in forensic analysis.
Answer_________
Uses are air pressure to force analytes and solvents through a long pad of the same material used in TLC plates. Capable of separating grams of compound.
Answer:___________________
Uses a high pressure pump to force analytes and solvent through micron size stationary phase material. Depending on the diameter of the column used, allows to monitor a reaction mixture or to separate mg (prep-column) of compound. A = pump, B = Injector, C =solvents, D = guard column, E = chromatography column, F = UV-Vis detector, G = computer readout, H = helium to degas the solvents
Answer:______Uses a large glass backed TLC plate and a streak of mixture at the origin line to separate out mg of compound after developing the plate, scrapping off the stationary phase, and extracting out the desired compound.
Answer:_________Uses the same material as used in TLC plates and centrifugal force to collect up to a gram of compound on the edge of its plate.
Answer:_______Explanation / Answer
Used to separate volatile liquids by boiling point, affinity to the stationary phase or even size (depending on the column type), this chromatographic technique can be used to monitor reactions or even to separate the components of complex aromas so that a human can smell them at the outlet (pictured top-center left). This technique is often used in forensic analysis.
Answer-
Gas Chromatography
Uses are air pressure to force analytes and solvents through a long pad of the same material used in TLC plates. Capable of separating grams of compound.
Answer-
Flash Chromatography
Uses a high pressure pump to force analytes and solvent through micron size stationary phase material. Depending on the diameter of the column used, allows to monitor a reaction mixture or to separate mg (prep-column) of compound. A = pump, B = Injector, C =solvents, D = guard column, E = chromatography column, F = UV-Vis detector, G = computer readout, H = helium to degas the solvents
Answer-
High Performance Liquid Chromatography
Uses a large glass backed TLC plate and a streak of mixture at the origin line to separate out mg of compound after developing the plate, scrapping off the stationary phase, and extracting out the desired compound.
Answer-
Preparative Chromatography
Uses the same material as used in TLC plates and centrifugal force to collect up to a gram of compound on the edge of its plate.
Answer-
Radial Chromatography
Used to separate volatile liquids by boiling point, affinity to the stationary phase or even size (depending on the column type), this chromatographic technique can be used to monitor reactions or even to separate the components of complex aromas so that a human can smell them at the outlet (pictured top-center left). This technique is often used in forensic analysis.
Answer-
Gas Chromatography
Uses are air pressure to force analytes and solvents through a long pad of the same material used in TLC plates. Capable of separating grams of compound.
Answer-
Flash Chromatography
Uses a high pressure pump to force analytes and solvent through micron size stationary phase material. Depending on the diameter of the column used, allows to monitor a reaction mixture or to separate mg (prep-column) of compound. A = pump, B = Injector, C =solvents, D = guard column, E = chromatography column, F = UV-Vis detector, G = computer readout, H = helium to degas the solvents
Answer-
High Performance Liquid Chromatography
Uses a large glass backed TLC plate and a streak of mixture at the origin line to separate out mg of compound after developing the plate, scrapping off the stationary phase, and extracting out the desired compound.
Answer-
Preparative Chromatography
Uses the same material as used in TLC plates and centrifugal force to collect up to a gram of compound on the edge of its plate.
Answer-
Radial Chromatography
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