in assorted Prelab Question #2: It is important to understand that while the abs

Question

in assorted Prelab Question #2: It is important to understand that while the absorbance value is related to the amount of light absorbed by a sample, it is not equal to or directly proportional to the amount of light absorbedConsider a situation where 100 photons of light pass through a sample. After the light passes through the sample, 20 photons are detected a) How many photons were transmitted through the sample? How many were absorbed by the sample? b) Calculate the % of photons transmitted and the % of photons absorbed. c) Use equation to the above calculate the absorbance of the solution from the % transmittance. which of the 100 . the ratio of their absorbance values. d) Now, calculate the absorbance of a solution in 10 photons are detected e) Compare the ratio of the % photons absorbed to

Explanation / Answer

a) Photon passed = 100

photons detected = 20

Photon transmitted = 20

photon Absorbed = 100 - 20 = 80 photons

b) photons transmitted = 20%

% photons absorbed = 80%

c) A = Iog10[100%/T]

= log10[100/20]

= 0.6990

d) A = log10(100/10)

= 1

e) ratio of % photons absorbed = 90/80 = 1.125

ratio of absorbance = 1/0.6990 = 1.431

both values are not equal

Therefore,

Absorbance value is not equal to or diectly proportional to the amount of light absorbed

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