The amino-acid sequence shown in the following table was obtained from the centr

Question

The amino-acid sequence shown in the following table was obtained from the central region of a particular polypeptide chain in the wild-type and several mutant bacterial strains: Each mutant represents a single nucleotide change from the original wild-type gene. For each mutant, explain what SINGLE mutation at the DNA level accounts for the change(s) in amino acid sequence Also, state in which codon and which position the mutation took place and then lulls characterize the mutation as a base-pair substitution mutation (transversion or transition, and missense. nonsense, etc ) or a frameshift mutation. (p. 104). 6.7 (p 108), and 7.3 (p. 133) will prove useful. Finally recreate as much of the original wild-type mRNA sequence as possible.

Explanation / Answer

Mutant 1: mutation in codon 3: proline-histidine. But as it changes all the rest of the seq, it is either addition/deletion

Mutant2:mutation in codon 5 changes in 5th codon onwrads. same as codon 3 addition/deletion

Mutant3:last codon has changed to a stop. base sustitution, non-sense

Mutant4: codon 5 has changed to a stop. base sustitution, non-sense

Mutant 5: codon 4 has changed to Ser. base substitution, sense

Wild type RNA: AAGGACGGGU GGCACUGGUG GUCCACC

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