magine you need to pour an SDS-PAGE gel in order to look for band shifts of your
ID: 54641 • Letter: M
Question
magine you need to pour an SDS-PAGE gel in order to look for band shifts of your protein (in the presence and absence of phosphatase). The protocol you have says to pour a 10 mL gel with 10% polyacrylamide, 0.1% SDS, and .06% ammonium persulfate (a reagent that aids in polymerization of the polyacrylamide into a gel). On the shelf in your lab you have stock solutions of 30% acrylamide, 0.4% SDS, and 10% ammonium persulfate. How much of each will you add to your gel-making solution? magine you need to pour an SDS-PAGE gel in order to look for band shifts of your protein (in the presence and absence of phosphatase). The protocol you have says to pour a 10 mL gel with 10% polyacrylamide, 0.1% SDS, and .06% ammonium persulfate (a reagent that aids in polymerization of the polyacrylamide into a gel). On the shelf in your lab you have stock solutions of 30% acrylamide, 0.4% SDS, and 10% ammonium persulfate. How much of each will you add to your gel-making solution? magine you need to pour an SDS-PAGE gel in order to look for band shifts of your protein (in the presence and absence of phosphatase). The protocol you have says to pour a 10 mL gel with 10% polyacrylamide, 0.1% SDS, and .06% ammonium persulfate (a reagent that aids in polymerization of the polyacrylamide into a gel). On the shelf in your lab you have stock solutions of 30% acrylamide, 0.4% SDS, and 10% ammonium persulfate. How much of each will you add to your gel-making solution?Explanation / Answer
Volume of acrylamide -Stock - 30% acrylamide - To get 10% acrylamide 3.3ml of stock + 6.7ml of wate
Volume of SDS - Stock- 0.4% - To get 0.1% 2.5ml of stock + 7.5 ml of water
Ammonium persulfate - Stock - 0.06% = We cannot make 10% ammonium sulfate from 0.06% since the stock concentration is lesser than the desired concentration.
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