39. Riboflavin (Vitamin B) is determined in a cereal sample in 5% acetic acid so
ID: 549420 • Letter: 3
Question
39. Riboflavin (Vitamin B) is determined in a cereal sample in 5% acetic acid solution. A calibration curve was prepared by measuring the fluorescence intensities of a series of standards of increasing concentrations. The following data were obtained by measuring the fluorescence intensity Riboflavin (ug/mL) Fluorescence Intensity 0.000 0.100 0.200 0.400 0.800 0.0 5.8 12.2 22.3 43.3 (a) Use the method of least squares to obtain the best straight line for the calibration curve and to calculate the concentration of riboflavin in the sample solution. The sample fluorescence intensity was 15.4. Include the /mL Riboflavin concentration: (b) Determine the uncertainty in the () slope, (i) intercept, (ii) y, and (iv) the riboflavin concentration. Sm. Su:Explanation / Answer
Riboflavin
Fluorescence intensity
0
0
0.1
5.8
0.2
12.2
0.4
22.3
0.8
43.3
The concentration of riboflavin = 0.3µg/ml
Riboflavin
Fluorescence intensity
0
0
0.1
5.8
0.2
12.2
0.4
22.3
0.8
43.3
Related Questions
Hire Me For All Your Tutoring Needs
Integrity-first tutoring: clear explanations, guidance, and feedback.
Drop an Email at
drjack9650@gmail.com
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.