1) Classify the following enzymes based on the descriptions of their activities

Question

1) Classify the following enzymes based on the descriptions of their activities (6 pts total) a) glucokinase; adds phosphate group (from ATP) to glucose b) aldolase; splits aldols into aldehyde and alcohol c) trypsin; cleaves peptide bonds through addition of water d) aconitase; converts citrate to isocitrate e) cytochrome P450; reduces O, to incorporate O into substrates f aminoacyl tRNA synthetase; joins amino acids with their corresponding tRNA 2) A hasty biochemistry trying to construct a Michaelis-Menten curve student forgot to record what the substrate concentrations for his samples A-H. He did however write down the enzyme velocities measured for each sample (4 pts total): le Velocity 30 19 48 mol min While the student still has the samples available, the laboratory report is due in 30 minutes and there is no time to figure out the missing concentrations. a) How could the student estimate the maximal velocity (Vaas) from his data? What would be the value of Vmax? (2 pts) b) The student still needs to determine KM. If the student could determine the concentration of just one sample (say, by measuring the absorbance and using Beer's Law), which sample should he check? Why? (2 pts) 3) Use the kinetic parameters shown below to answer the following questions (4 pts total): a) which substrate binds more tightly? (2 pts) b) which substrate has higher specificity? (2 pts) 10 25

Explanation / Answer

1.

a) transferases ( transfer a phosphorous contaoning groups)

b) lyases

c)Hydrolases

d) lyase as  The mechanism of aconitase classifies it as a lyase, even if (under most physiological conditions), the relative concentrations of substrates results in it catalysing the conversion of citrate to isocitrate. In short, it is not an isomerase because the substrate is released after each step.

read more https://en.wikibooks.org/wiki/Structural_Biochemistry/Specific_Enzymes_and_Catalytic_Mechanisms/Enzyme_Classification

or

http://www.sbcs.qmul.ac.uk/iubmb/enzyme/rules.html

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