I was having a hard time figuring out this question for geneticscould someone pl

Question

I was having a hard time figuring out this question for geneticscould someone please help?
You have grown bacteria in a liquidculture and would like to know the titer of that bacterial culture.You first make a 100 fold dilution of your culture followed by a 2fold dilution, and then you spread 2 ml of the final dilutedsolution onto a plate of medium. 25 colonies grow on the plate.What was the titer of the original liquid culture? Describe how youwould best make the two dilutions.
You have grown bacteria in a liquidculture and would like to know the titer of that bacterial culture.You first make a 100 fold dilution of your culture followed by a 2fold dilution, and then you spread 2 ml of the final dilutedsolution onto a plate of medium. 25 colonies grow on the plate.What was the titer of the original liquid culture? Describe how youwould best make the two dilutions.

Explanation / Answer

If I am interpreting the question correctly, they are basicallyasking what dilution factor can be applied to the original liquidculture and still have at least one bacteria available toinfect. If so, the 100 fold dilution followed by a 2 folddilution is equivalent to a 200 fold dilution.   Since 25colonies grew, the solution could have been diluted another 25 foldto still yield a single bacteria. So the titer should be1:5000 [200*25]. To prepare these dilutions you would 1ml ofthe original sample and add 99ml of water. Then you would add50ml of that sample followed by another 50ml of water. Hope that helps

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