The following data represents the oxygen binding activity of purified myoglobin,
ID: 55440 • Letter: T
Question
The following data represents the oxygen binding activity of purified myoglobin,
purified hemoglobin, and hemoglobin in human blood cells.
pO2 (Torr)
Fraction of purified myoglobin with O2
pO2(Torr)
Fraction of purified hemoglobin with O2
pO2(Torr)
Fraction of Hemoglobin in blood with O2
0.5
0.161
0.1
.00315
10.6
0.10
1.0
0.277
0.35
.0099
19.5
0.30
2.0
0.434
0.794
.0306
27.4
0.50
3.0
0.535
1.748
.0909
37.5
0.70
4.0
0.605
2.884
.24
50.4
0.85
6.0
0.697
4.467
.50
77.3
0.96
8.0
0.754
5.75
.76
92.3
0.98
12
0.821
7.94
.909
20
0.885
12.88
.969
29.51
.99
67.60
.997
A) Construct the oxygen binding curves of purified myoglobin and hemoglobin and
hemoglobin in blood cells and estimate p50 of myoglobin and hemoglobin under these conditions.
B) Estimate Hill coefficient (n) of purified hemoglobin and hemoglobin in blood cells.
pO2 (Torr)
Fraction of purified myoglobin with O2
pO2(Torr)
Fraction of purified hemoglobin with O2
pO2(Torr)
Fraction of Hemoglobin in blood with O2
0.5
0.161
0.1
.00315
10.6
0.10
1.0
0.277
0.35
.0099
19.5
0.30
2.0
0.434
0.794
.0306
27.4
0.50
3.0
0.535
1.748
.0909
37.5
0.70
4.0
0.605
2.884
.24
50.4
0.85
6.0
0.697
4.467
.50
77.3
0.96
8.0
0.754
5.75
.76
92.3
0.98
12
0.821
7.94
.909
20
0.885
12.88
.969
29.51
.99
67.60
.997
Explanation / Answer
A) P50 is that pO2 (Torr) where the fraction of the protein ( here myoglobin and hemoglobin) with 0.5 or 50% O2.
Thus, from the above data, P50 for myoglobin = 2.7
and P50 for hemoglobin = 4.467
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