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QUESTION 24 5\'-end of coding strand 5\'-end of template strand 3\'-end of antis

ID: 55474 • Letter: Q

Question

QUESTION 24

5'-end of coding strand

5'-end of template strand

3'-end of antisense strand

5'-end of RNA

3'-end of sense strand

3'-end of nonsense strand

Are you kidding me?

5

2

1

6

4

3

3.5 points   

QUESTION 25

In Fig 2, where do you expect to find the most important promoter elements?

Near 5 (and 6)

Near 1 (and 2)

Near 3

Near 4

3.5 points   

QUESTION 26

Transcription termination in E. coli  

depends conserved sequences on the DNA to which a Rho factor binds preventing further forward movement of the polymerase (Rho-dependent mechanism) or requires the polyemerase to encounter another promoter to which a Sigma factor is bound Rho-independent mechanism).

either requires a a Rho factor to actively pull the mRNA off the DNA (Rho-dependent mechanism) or depends on sigma factor accomplishing essentially the same thing (sigma-dependent mechanism)  .

either requires the Rho factor (Rho-dependent mechanism) or depends on the RNA polymerase working in reverse (Rho-independent mechanism).

either requires the Rho factor to actively pull the mRNA off the DNA (Rho-dependent mechanism) or depends on a stem loop structure followed by an AU-rich region in the mRNA causing its dissociation from the DNA (Rho-independent mechanism).

3.5 points   

QUESTION 27

Which of the following best characterizes the average rates and error frequencies of DNA synthesis and RNA synthesis in vivo?

The rate of transcription increases when the error frequency of DNA replication increases.

The rate of transcription decreases when the error frequency of DNA replication increases.

Transcription is slower and more error-prone than DNA replication.

Transcription is faster and less error-prone than DNA replication.

Transcription is slower and less error-prone than DNA replication.

Transcription is faster and more error-prone than DNA replication.

3.5 points   

QUESTION 28

Which of the following most accurately describes the relative diversity (i.e., number of different transcripts) of RNA species inside cells?

rRNA < tRNA < mRNA

tRNA < rRNA < mRNA

mRNA < rRNA < tRNA

mRNA < tRNA < rRNA

tRNA < mRNA < rRNA

3.5 points   

QUESTION 29

Which of the following sequences would interact with RNA polymerase holoenzyme that contain a ?70 subunit most efficiently?

        –35                 –10          +1
5' …GAGCCC …… CACTTT… Start Site... 3'

         –35                 –10          +1
5' …TTGACT …… TATAAA… Start Site... 3'

        –20              –10        +1
5' …TAAGAA … CACAAT… Start Site....3'

     –20            –10          +1
5' …TTGACA … TATAAT… Start Site... 3'

    –35                  –10           +1
5' …ACAAGA …… CACGTT… Start Site... 3'

      -       A.       B.       C.       D.       E.       F.       G.   

5'-end of coding strand

Read Answer Items for Question 24       -       A.       B.       C.       D.       E.       F.       G.   

5'-end of template strand

Read Answer Items for Question 24       -       A.       B.       C.       D.       E.       F.       G.   

3'-end of antisense strand

Read Answer Items for Question 24       -       A.       B.       C.       D.       E.       F.       G.   

5'-end of RNA

Read Answer Items for Question 24       -       A.       B.       C.       D.       E.       F.       G.   

3'-end of sense strand

Read Answer Items for Question 24       -       A.       B.       C.       D.       E.       F.       G.   

3'-end of nonsense strand

Read Answer Items for Question 24 Answer A.

Are you kidding me?

B.

5

C.

2

D.

1

E.

6

F.

4

G.

3

Explanation / Answer

25. Near 5 ( it represents the 3' end of antisense strand)

26. Either requires a Rho factor to actively pull the mRNA off the DNA or depends on a stem loop strucutre followed by an AU rich region in the mRNA causing its dissociation from the DNA (intrinsic or Rho-independent mechanism).

27. C. Transcription is slower (20 times slower) and more error prone than DNA replication

28. A. rRNA<tRNA<mRNA

29. B. 5'...TTGACT(-35).......TATAAA(-10)......3'

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