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Hepatitis delta virus (HDV) has an unusual genomic structure and mode of replica

ID: 55532 • Letter: H

Question

Hepatitis delta virus (HDV) has an unusual genomic structure and mode of replication. Given what you know of HDV, fill in the following table and explain your answer.

Genomic and antigenomic RNA synthesis and mRNA transcription

Characteristic

Genomic Synthesis

Antigenomic Synthesis

mRNA transcription

1

RNA polarity (+ or -)

2

Sensitivity to a-amanitin (yes or no)

Yes

Yes

3

Small delta antigen requirement (yes or no)

4

Polymerase

5

Site of synthesis

Nucleoplasm

Nucleolus

Nucleoplasm

Characteristic

Genomic Synthesis

Antigenomic Synthesis

mRNA transcription

1

RNA polarity (+ or -)

2

Sensitivity to a-amanitin (yes or no)

Yes

Yes

3

Small delta antigen requirement (yes or no)

4

Polymerase

5

Site of synthesis

Nucleoplasm

Nucleolus

Nucleoplasm

Explanation / Answer

Characteristic

Genomic Synthesis

Antigenomic Synthesis

mRNA transcription

RNA polarity (+ or -)

Positive

Negative

Positive

Sensitivity to a-amanitin (yes or no)

Yes

Yes

No

Small delta antigen requirement (yes or no)

Yes

No

Yes

Polymerase

Takes place in nucleoplasm, mediated by RNA pol II

Occurs in nucleus, mediated by RNA pol I

occurs in nucleus, mediated by RNA pol II

Site of synthesis

Nucleoplasm

Nucleolus

NucleoplasmBottom of Form

Characteristic

Genomic Synthesis

Antigenomic Synthesis

mRNA transcription

RNA polarity (+ or -)

Positive

Negative

Positive

Sensitivity to a-amanitin (yes or no)

Yes

Yes

No

Small delta antigen requirement (yes or no)

Yes

No

Yes

Polymerase

Takes place in nucleoplasm, mediated by RNA pol II

Occurs in nucleus, mediated by RNA pol I

occurs in nucleus, mediated by RNA pol II

Site of synthesis

Nucleoplasm

Nucleolus

NucleoplasmBottom of Form

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