In order to characterize the promoter region of your favorite gene (YFG), you ha

Question

In order to characterize the promoter region of your favorite gene (YFG), you have cloned a 200

nucleotide piece of genomic DNA upstream of the transcriptional start site and will use it to drive

expression of the chloroamphenicol acetyltransferase (CAT) reporter in mammalian cells growing in

culture. You have also deleted various portions of the 5’ end of the cloned piece and have tested

those constructs for CAT activity as well (left panel below). The results of CAT activity for constructs

1 through 5 are expressed in the graph (right panel below):

For the following questions, answer A, B, C, D or any combination.

a. What is the minimal promoter region (or regions) required for maximal expression?

b. What region of the promoter contains a repressor binding site?

c. What region of the promoter is dispensable for normal YFG regulation?

d. In which region would mutations be most tolerated?

Explanation / Answer

You have not provided me the graph to answer this question. Could you please give the details of graph so that I can answer this question efficiently.

Related Questions

Navigate

• Browse (All)
• Browse I
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.