24) Refer to the bisubstrate mechanism schemes shown below. Correct description.

Question

24) Refer to the bisubstrate mechanism schemes shown below. Correct description. CHM 380 (Fall 2017) Exam 2 answer pairs scheme with correct E'BEQ Scheme I E AE Scheme 2 Which type of scheme is not typically accompanied by burst kinetics? a) Scheme 1; single-displacement b) Scheme 1; double-displacement (ping pong) c) Scheme 2; single-displacement d) Scheme 2; double-displacement (ping pong) Questions 25-27: Classify the following enzymes using the classifications we discussed in class.. Choices for Q. 25-27 a) hydrolase b) isomerase c) lyase d) ligase e) oxidoreductase 25) catalase: oxidizes and reduces hydrogen peroxide to dioxygen (O) and water 26) CcrA metallo--lactamase: uses water to break lactam bond in -lactam compounds 27) rotamase A: converts proline from cis to trans form (and vice versa) lo

Explanation / Answer

24)

scheme 1 = double-displacement (ping-pong)

scheme 2 = single displacement

double-displacement (ping-pong) :

- It is a 2 stage reaction.

single displacement:

It is also called sequential reaction.

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Burst kinetics is not accompanied by double-displacement (ping-pong) reactions i.e. scheme 1.

Burst kinetics means On adding enzyme to the substrate a large initial velocity is exhibited and enzyme velocity linearly increases.

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