How can I create an experiment testing the effects of low pH on enzyme activity?

Question

How can I create an experiment testing the effects of low pH on enzyme activity?

Help! I’m supposed to do an experiment, but I’m kind of confused. I have five test tubes, and I’m supposed to be testing the effects of low pH on enzyme activity. I’ve chosen Amylase/banana as my enzyme and starch/potato as my substrate. (I also have left over starch liquid and IKI.) I was going to test the different pHs by adding water (7), draino (14), soda (4), banana (5) and soda (4) to 1 cubic centimeter of a potato in each tube. I guess I’m just not sure if I’m doing it right.

The independent variable influences the change in my dependent variable. Is my dependent variable the potato? My independent variable would be the water, draino, soda, banana and soda. What would my control be? Thank you for your help.

Explanation / Answer

Here, the potato starch is the substrate, so that it is the dependent variable. Now, your independent variables are with different pH's, but the source of enzyme amylase is a banana. Therefore, the banana independent variable containing test tube will be your control. By negotiating that banana independent variable test tube containing enzyme activity, you can measure the amylase activity at other independent variables of different pH's containing test tubes.

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