Prokaryotic DNA Pol III cannot catalyze nick translation. Why? a. It lacks 3\'-5
ID: 558029 • Letter: P
Question
Prokaryotic DNA Pol III cannot catalyze nick translation. Why? a. It lacks 3'-5' exonuclease activity b. It lacks 5'-3' exonuclease activity c. It lacks 3'-5' endonuclease activity e. It lacks 5'-3' endonuclease activity e. none of the above
Which of the following enzyme activity enables DNA Pol I to edit its mistakes? a. 3'-5' exonuclease activity b. 5'-3' exonuclease activity c. 3'-5' endonuclease activity d. 5'-3' endonuclease activity e. none of the above
How is mRNA translated? a. 5' 3' direction b. 3' 5' direction c. amino-to-carboxyl direction d. carboxyl-to-amino direction e. none of the above
Which of the following processes is not a part of gene expression? a. replication b. transcription c. processing of mRNA d. translation e. none of the above
Explanation / Answer
1) b. It lacks 5'-3' exonuclease activity
Prokaryotic DNA Pol III cannot catalyze nick translation, because it lacks 5'-3' exonuclease activity. Prokaryotic DNA Pol I catalyze nick translation due to 5' -3' exonuclease activity.
Which of the following enzyme activity enables DNA Pol I to edit its mistakes?
2) a. 3'-5' exonuclease activity
In, prokaryotic all three DNA polymerases have the ability to proofread, using 3’-> 5’ exonuclease activity. The 3'-> 5' exonuclease activity of DNA polymerase I proofreads newly synthesized DNA, removing mismatched nucleotides.
3) c. amino-to-carboxyl direction
The sequence of nucleotides in the template
m- RNA chain determines the sequence of amino acids, which are generated in amino acid chain. Addition of an amino acid occurs at the C-terminus of the peptide and thus translation is said to be amino-to-carboxyl directed.
4) a. Replication
Replication is not a part of gene expression. It is a part of cell division. It is a process that happens when DNA needs to be divided in mitosis.
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