Use the double-stranded DNA molecule below :o answer the following questions The

Question

Use the double-stranded DNA molecule below :o answer the following questions The first pa r of nucleotides (circled) is the start point of transcription. Write the correct mRNA produced from the transcription of this DNA molecule. Indicate the corresponding 5' and 3' ends of the mRNA molecule. Reference a genetic code table to write the sequence of the polypeptide assembled by the translation of the mRNA produced by the transcription of the DNA molecule shown above. indicate the amino and the carboxyl ends.

Explanation / Answer

The DNA sequence is, 5'- ATGATCGGATCGATCCAT-3'.

(a) Its mRNA sequence is, 5'- AUGAUCGGAUCGAUCCAU-3'. (RNA contains uracil in place of thymine).

(b) The amioacid sequence produced by the 5'- AUG AUC GGA UCG AUC CAU-3' is,

Amino--- Met-Ile-Gly-Ser-Ile-His---------Carboxy. (AUG that codes for methionine is the start codon).

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