1) Prove to me, with structures, why I can’t base pair with G! And I=inosine, no
ID: 55941 • Letter: 1
Question
1) Prove to me, with structures, why I can’t base pair with G! And I=inosine, not “me!”
2) Below is sequence taken from the 5’ end of a prokaryotic mRNA. Underline the probable Shine-Delgarno
sequence and then tell me the first four amino acids (N-term --> C-term) of the protein produced from this mRNA!
5'....auacaaaacauugagaaauguggcggcggauaugguuucagccggaauag....3'
3) Given the structures below, draw the curved arrow mechanism showing how water releases a finished protein from the ribosome protein synthesis complex. You don’t need to know what is going on in the active site of the ribosome, what else is involved, or etc. Just show what should be done to make the water do this:
fMet Protein OH Final amino acid Final tRNAExplanation / Answer
1)
Based on the tRNA base pairing rule, the G only binds to U and C and the I binds to A, C and U except G. This is because of both Guanine and Inosine are structurally similar, they have same nucleobase structure with small difference. Hence, due to stereochemical hindrance the “I” and G cannot pair each other (Wobble base pair).
2)
Shine-Delgarno sequence is present in mRNA, which located 8 base pairs upstream to +1 and complimentary to the rRNA sequence in Ribosomes. So, ribosomes directly bind on the Shine-Delgarno sequence and begin the protein synthesis. The conserved sequence of Shine-Delgarno sequence is “AGGA”
So, the Shine-Delgarno sequence in the given mRNA is probably:
5'....auacaaaacauugagaaauguggcggcggauaugguuucagccggaauag....3'
The First four amino acids (N-term --> C-term) of the protein produced from this mRNA are: MWRR.
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