help please Describe the structure/function of the parts of nucleus: Nuclear env
ID: 55951 • Letter: H
Question
help please Describe the structure/function of the parts of nucleus: Nuclear envelope Nucleoplasm Nuclear lamina Nuclear pore Nucleolus Define: Nudeosome Solenoid or Thickfiber Loop Chromatin Chromosome Euchromatin Heterochromatin What are the two dyes used for staining in this experiment and what so they stain? What is function of SDS and 0.5M NaCI in the isolation of DNA How did DNase affect the spooling of DNA? What % of ethanol was used in the DNA isolation and where did stand when added to aqueous solution and why? How did the chromatin in the nucleus appear after the addition of SDS, DNAse and 0.5M NaCI Centrifugation: What are the different methods used for breaking the cells open? What is the relation between RPM and RCF? What RCF is used for the separation of nuclei, mitochondria, microsomes and ribosomes? Calculate the RCF if the revolution per minute of the rotor is 1000 and the radius is 100mm. Calculate the RPM if the radius of the rotor is 75mm and the RCF is 550 xG What type of centrifuge, rotor and tubes did you use for nuclei separation? What necessary step do you need to take before keeping the centrifuge tubes in the rotor and starting the centrifugation?Explanation / Answer
Nucleosome: Arrangement of DNA coiled around 8 histone proteins.
Solenoid: Nucleosomes coil into much smaller space and form 30 nm solenoid.
Loop: Next level of compressed DNA, which has solenoids coiled into small turns.
Chromatin: Loops are compressed more with the help of more proteins, so that it can fit inside the nucleus.
Chromosome: Thread like structures seen at metaphase, maximum compressed form of DNA.
Heterochromatin: Tightly packed areas of DNA which are not in access to enzymes.
Euchromatin: Active DNA form which is lightly packed so that enzymes can access the genes for translation and transcription.
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