118 Laboratory Manwal for General, Organic and Biological Chemistry Q3 What subs
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118 Laboratory Manwal for General, Organic and Biological Chemistry Q3 What substancets) were retained inside the dialysis bag? Why were they retained? C. Filtration 1. Appearance of filter paper 2. Substance present on filter paper Substance Present Observations 3. Identification Test CI Test Starch Test Glucose Test 4. Substance Solution, Colloid, Suspension? Reason CI Starch Glucose Charcoal Questions and Problems Q4 State whether each of the following is isotonic, hypotonic, or hypertonic: a. H2O b. 0.9% (m/v) NaCl c. 10% (m/v) glucose d. 3% (m/v) NaCl e. 0.2% (m/v) NaCl 05 Predict the effect on a red blood cell (crenation, hemolysis, or none) that the following solutions would have: a. 2% (m/v) NaCl b. H2O c. 5% (m/v) glucose d. 1% (m/v) glucose e. 10%(n/v) glucose Copyright © 2014 Pearson Education, Inc.Explanation / Answer
Q. 3: Dialysis bags are used to separate the organic compounds based on molecular weight. Each dialysis bags have specific pore size eg. 1000 dalton. This 1000 dalton dialysis bag can separate organic compound which have less molecular weight than 1000 dalton and more than 1000 dalton will remain inside the dialysis bags.
Q. 4: 5.0% (m/v) glucose and 0.9 % NaCl are isotonic soltion to human blood cells.
a. H2O hypertonic
b. 0.9% (m/v) NaCl Isotonic
c. 10% (m/v) glucose hypertonic
d 3 % (m/v) NaCl hypertonic
e 0.2% (m/v) NaCl hypotonic
Q. 5 if RBC placed in hypertonic solution then it start shrinking. the process is called crenation. If it placed in hypotonic solution then the cells starts swelling and finally it brusts, the process is called hemolysis. In isonic solution nothing happens to cell.
a. 2% (m/v) NaCl creanation
b. H2O hemolysis
c. 5% (m/v) glucose none
d. 1% (m/v) glucose hemolysis
e. 10% (m/v) glucose crenation
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